Detection of <i>Clostridium perfringens</i> Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System.

ABSTRACT: Clostridium perfringens is a highly versatile pathogen of humans and animals. Rapid and sensitive detection methods for C. perfringens are urgently needed for the timely implementation of control. In this study, to provide novel promising methods for the detection of C. perfringens, two rapid, sensitive, and instrument-free C. perfringens detection methods based on recombinase-aided amplification (RAA) assay and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 12a (CRISPR/Cas12a) system were developed depending on fluorescence signal (RAA-CRISPR/Cas12a-FL) and lateral flow strip (RAA-CRISPR/Cas12a-LFS), respectively. The limit of detection of the RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12a-LFS methods is 2 copies an

SUBMITTER: Xiao X 

PROVIDER: S-EPMC12016868 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature