Isolation and Genomic Characterization of the G6P[1]-Type Sheep Rotavirus in China.

ABSTRACT: Rotavirus A (RVA) is a prevalent cause of enteric diarrhea in infants, bovine, pigs, and sheep globally. Currently, the G6P[1]-type rotaviruses are prevalent in sheep or goat in Bangladesh, Turkey, and Uganda. However, this genotype has not been reported in Chinese sheep or goat. Therefore, 12 anal swabs were collected from diarrheal sheep in Gansu Province, China, in 2023 and tested for rotavirus using reverse transcription-polymerase chain reaction (RT-PCR). Pathological sections and immunohistochemistry were used to observe pathological changes and rotavirus antigens in the duodenum, respectively. The sheep rotavirus was isolated in MA-104 cells and characterized through indirect immunofluorescence and transmission electron microscopy. The genes of the strain were obtained using the nex

SUBMITTER: Li P 

PROVIDER: S-EPMC12016906 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature