Genetic Characterization of <i>Canine morbillivirus</i> (Canine Distemper Virus) Field Strains in Dogs, Chile, 2022-2023.

ABSTRACT: Canine distemper virus (CDV) poses a significant threat to dogs and wildlife worldwide, and this study sought to provide an updated genetic characterization of CDV field strains in Chile during 2022-2023. We collected samples from 52 suspected CDV cases in domestic dogs and detected viral RNA through real-time RT-PCR in 28 dogs (54%). Factors like age and vaccination status were determinants of CDV positivity, with young adult and unvaccinated dogs facing a higher infection risk. We isolated CDV from positive samples in VeroDogSLAM cells. From these isolates and direct samples, we obtained sequences and estimated the phylogeny based on gene H. CDV isolates from nasal and conjunctival swabs exhibited cytopathic effects, and sequence analysis unveiled a substantial genetic diversity among th

SUBMITTER: Ariyama N 

PROVIDER: S-EPMC12017005 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature