Propidium Monoazide Integrated With qPCR Enables Rapid and Universal Detection of Infectious Porcine Reproductive and Respiratory Syndrome Viruses.

ABSTRACT: Infectious porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS, but noninfectious PRRSV cannot. PCR and ELISA are commonly used for PRRSV detection but they cannot discriminate PRRSV infectivity. Virus isolation is a gold standard to determine virus infectivity. However, it is time-consuming. Therefore, we developed a propidium monoazide (PMA) qPCR assay for rapid and universal detection of infectious PRRSV in this study. After comparing the inactivation efficacies of distinct disinfectants, ultraviolet (UV) light, and heat, heat at 72°C for 15 min was determined as an effective strategy for PRRSV inactivation, which was confirmed by virus isolation and immunofluorescence assay (IFA) detection. In addition, PMA pretreatment parameters were optimized, including PMA conce

SUBMITTER: Qi W 

PROVIDER: S-EPMC12017026 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature