An RT-qPCR Assay from Rectal Swabs for the Detection of Rabbit Hemorrhagic Disease Virus 2 in Natural Cases.

ABSTRACT: Rabbit hemorrhagic disease virus 2 (RHDV2 or Lagovirus europaeus GI.2) is spreading across North America. This has enabled submissions of lagomorphs for testing to veterinary diagnostic laboratories (VDLs). The liver is currently the gold-standard sample type for testing by RT-qPCR. VDL clients usually seek alternate diagnostic approaches that permit simpler and faster sample collection with less risk of environmental contamination; there is also a necessity for a sample type that can be collected from live animals. Therefore, the goal of this study was to optimize and evaluate an RT-qPCR assay on rectal swabs collected from a group of carcasses of leporids of different species that were submitted to a VDL during an RHDV2 outbreak. A total of 130 carcasses were tested both by liver

SUBMITTER: Asin J 

PROVIDER: S-EPMC12017109 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature