Interferon-γ and IL-27 positively regulate type 1 regulatory T cell development during adaptive tolerance.

ABSTRACT: Strong T cell receptor (TCR) and interleukin (IL)-27 signaling influence type 1 regulatory (Tr1) T cell development, but whether other signals determine their differentiation is unclear. Utilizing Tg4 TCR transgenic mice, we established a model for rapid Tr1 cell induction. A single high dose of [4Y]-MBP peptide drove the differentiation of Il10 ⁺ T cells with Tr1 cell mRNA and protein signatures. Kinetic transcriptional and phenotypic analyses revealed that the Tr1 cell module was transient and preceded by Ifng transcription in other CD4⁺ T cells. Changes in Tr1 cell frequency correlated with altered macrophage activation, while neutralization of interferon (IFN)γ reduced Tr1 cell frequency and the TCR signal strength markers Nur77, inducible T cell cos

SUBMITTER: Lecky DAJ 

PROVIDER: S-EPMC12018090 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature