Systemic inflammatory responses to repeated and increasing endotoxin challenges in fetal sheep.

ABSTRACT: Repeated low-dose administration of lipopolysaccharide (LPS) attenuates subsequent fetal responses, which makes it challenging to investigate interventions to prolonged exposure. Our aim was to develop a fetal inflammatory response syndrome (FIRS) model that consistently and effectively elicits a marked physiological response to increasing LPS doses. Four intravenous LPS boluses (0.3, 1.5, 3, and 15 μg) were administered to fetal sheep over 5 days. Physiological responses were measured via blood gases, pH, lactate, and cortisol concentrations. Fetal peripheral blood mononuclear cells (PBMCs) were analyzed for transcriptomic changes and tissue cytokine expression postmortem. All LPS challenges increased lactate, cortisol, and pCO₂ concentrations and decreased pH and pO₂

SUBMITTER: Kathuria S 

PROVIDER: S-EPMC12018166 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature