Acute myeloid leukemia-derived extracellular vesicles induced DNA methylation changes responsible for inflammatory program in normal hematopoietic stem progenitor cells.

ABSTRACT:

Introduction

Acute Myeloid Leukemia (AML) cells communicate with surrounding normal cells, including hematopoietic stem progenitor cells (HSPCs), in the bone marrow, and modify their fate supporting tumor growth. This communication can be mediated by Extracellular Vesicles (EVs), small vectors carrying a range of tumor molecular information. One of the hallmarks of AML is the aberrant DNA methylation. It is not known if and how AML cells can modify the epigenomic profile of healthy HSPCs. Here, we investigated the DNA methylation profile of HSPCs after exposure to AML derived-EVs.

Methods

Cord blood derived-HSPCs were treated with AML cell line derived-EVs for 20 hours and then their DNA methylation profile was analyzed by methylation array. We cross-referenced differential

SUBMITTER: Lamorte D 

PROVIDER: S-EPMC12018244 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature