Rapid detection of drug resistance in <i>Mycobacterium tuberculosis</i> clinical isolates for first-line antitubercular drugs by using a novel reporter mycobacteriophage.

ABSTRACT: The emergence of drug resistance presents a major challenge for the treatment of tuberculosis (TB). Reporter phage can provide an effective method for drug susceptibility testing (DST) of Mycobacterium tuberculosis (Mtb), but limited for parts of antitubercular drugs and the process of operation is usually time-consuming. Herein, we developed a new, sensitive, reporter phage with optimized method to detect drug susceptibility of Mtb clinical isolates for all first-line antitubercular drugs. The P _furAma promoter and nanoluciferase (Nluc) reporter sequences were integrated into the genome of the TM4 mycobacteriophage to generate a reporter phage, designated φFN. By optimizing concentration of Mtb, Tween 80 and drugs, we have established an effic

SUBMITTER: Guo M 

PROVIDER: S-EPMC12018339 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature