Bi-phasic integrated silk fibroin/polycaprolactone scaffolds for osteochondral regeneration inspired by the native joint tissue and interface.

ABSTRACT: Osteochondral scaffolds designed with bi-phasic and multi-phasic have typically struggled with post-implantation delamination. To address this issue, we developed a novel integrated scaffold with natural and continuous interface and heterogeneous bilayer structure. Through layer-by-layer wet electrospinning, two-dimensional (2D) bi-layer integrated membranes of silk fibroin (SF) and polycaprolactone (PCL) were fabricated. These membranes were then transformed into three-dimensional (3D) scaffolds using a CO₂ gas foaming technique, followed by gelatin coating on the osteogenic layer to afford final bi-phasic porous scaffolds. In vitro studies indicated that the 3D scaffolds better-maintained cell phenotypes than conventional 2D electrospun films. Additionally, the 3D scaff

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12018571 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature