The blood transcriptional response in patients developing intensive care unit-acquired pneumonia.

ABSTRACT:

Background

Immune response dysregulation has been implicated in the development of intensive care unit (ICU)-acquired pneumonia. We aimed to determine differences in the longitudinal blood transcriptional response between patients who develop ICU-acquired pneumonia (cases) and those who do not (controls).

Methods

We performed a case-cohort study in mechanically ventilated trauma and surgery patients with ICU stays >2 days, enrolled in 30 hospitals across Europe. We collected blood for RNA sequencing at baseline, day 7 and (in cases) the day of pneumonia diagnosis. We performed gene set enrichment analysis and analysed longitudinal gene expression changes using linear mixed models. External validation was performed using an independent trauma cohort.

Results

We enrolle

SUBMITTER: de Brabander J 

PROVIDER: S-EPMC12018758 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature