Structure-based virtual screening aids the identification of glycosyltransferases in the biosynthesis of salidroside.

ABSTRACT: Glycosylation plays an important role in the structural diversification of plant natural products. The identification of efficient glycosyltransferases is also a crucial step for the biosynthesis of valuable glycoside products. However, functional characterization of glycosyltransferases (GTs) from an extensive plant gene list is labour-intensive and challenging. Salidroside is a bioactive component derived from plants, widely utilized in the fields of food and medicine. Here, through transcriptome analysis and structure-based virtual screening, we identified two GTs that participated in the biosynthesis of salidroside from a rarely studied herbaceous plant, Astilbe chinensis. Ach15909 was found to possess high catalytic activity as evidenced by the determination of its catalytic parameter

SUBMITTER: Yao Y 

PROVIDER: S-EPMC12018814 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature