A split ribozyme system for in vivo plant RNA imaging and genetic engineering.

ABSTRACT: RNA plays a central role in plants, governing various cellular and physiological processes. Monitoring its dynamic abundance provides a discerning understanding of molecular mechanisms underlying plant responses to internal (developmental) and external (environmental) stimuli, paving the way for advances in plant biotechnology to engineer crops with improved resilience, quality and productivity. In general, traditional methods for analysis of RNA abundance in plants require destructive, labour-intensive and time-consuming assays. To overcome these limitations, we developed a transformative innovation for in vivo RNA imaging in plants. Specifically, we established a synthetic split ribozyme system that converts various RNA signals to orthogonal protein outputs, enabling in vivo visualisatio

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12018833 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature