Selection and validation of stable reference genes in potato infected by Pectobacterium atrosepticum using real-time quantitative PCR.

ABSTRACT: Potato blackleg disease is caused by Pectobacterium atrosepticum, can seriously destroy potatoes' growth and development. To accurately evaluate the expression levels of genes involved in potato (Solanum tuberosum) responses to P. atrosepticum infection, seven candidate reference genes (EF1α, eIF5A3, Tubulin, Ubiquitin, GAPDH, Actin, and CYP3) were systematically assessed for their expression stability at 1, 3, and 5 days after inoculation (dai) using the geNorm, NormFinder, BestKeeper, ∆Ct, and RefFinder algorithms. The results demonstrated that EF1α exhibited the highest stability among all experimental conditions, followed by eIF5A3 and Tubulin, whereas Ubiquitin displayed the least stability. To validate the screening outcomes, the expression patterns of four disease resistance-related

SUBMITTER: Hou L 

PROVIDER: S-EPMC12019324 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature