Chemometrics-Assisted Enhancement of Electrochemical Biosensor Performance toward miRNA Detection.

ABSTRACT: Chemometrics represents a potent tool for optimizing the experimental setup and subsequently boosting the performance of analytical methods. In particular, design of experiments (DoE) allows the experimental conditions to be optimized with high accuracy and a lower number of experiments when compared with the classical univariate approach, also known as one variable at a time (OVAT), which provides only a partial understanding on how factors affect the response. In this work, DoE was exploited, specifically a D-optimal design was used, to improve the analytical performance of a hybridization-based paper-based electrochemical biosensor, taking as target of the study the miRNA-29c (miR-29c) that is related to triple negative breast cancer. The sensing platform is composed of six variables to

SUBMITTER: Cimmino W 

PROVIDER: S-EPMC12019774 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature