Redox signaling modulates axonal microtubule organization and induces a specific phosphorylation signature of microtubule-regulating proteins.

ABSTRACT: Many life processes are regulated by physiological redox signaling, but excessive oxidative stress can damage biomolecules and contribute to disease. Neuronal microtubules are critically involved in axon homeostasis, regulation of axonal transport, and neurodegenerative processes. However, whether and how physiological redox signaling affects axonal microtubules is largely unknown. Using live cell imaging and super-resolution microscopy, we show that subtoxic concentrations of the central redox metabolite hydrogen peroxide increase axonal microtubule dynamics, alter the structure of the axonal microtubule array, and affect the efficiency of axonal transport. We report that the mitochondria-targeting antioxidant SkQ1 and the microtubule stabilizer EpoD abolish the increase in microtubule dy

SUBMITTER: Conze C 

PROVIDER: S-EPMC12019850 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature