Palm Multidiagnostic of <i>Mycoplasma pneumoniae</i>, <i>Chlamydia pneumoniae</i>, <i>Haemophilus influenzae</i>, and <i>Streptococcus pneumoniae</i> Using One-Tube CRISPR/Cas12a.

ABSTRACT: The recent high incidence of Mycoplasma pneumoniae (Mp) infections has raised widespread public health concerns. Therefore, rapid and accurate diagnosis of respiratory pathogenic microbial infections is of paramount importance to provide clinicians with accurate diagnostic insights and guide clinical medication. In response to this urgent need, we developed a one-tube Palm CRISPR/Cas12a Diagnostic (PaCD) method. This method facilitates the rapid detection of Mp infections, as well as three other prevalent respiratory pathogens, Chlamydia pneumoniae (Cp), Haemophilus influenzae (Hi), and Streptococcus pneumoniae (SP). In addition, 3D printing was employed to fabricate a compact detection device that includes a temperature control module set at 39°C and a blue lig

SUBMITTER: Kan X 

PROVIDER: S-EPMC12019929 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature