Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8⁺ T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice.

ABSTRACT:

Background

Processes related to how the intracranial microvasculature initiates brain‒peripheral crosstalk for subsequent blood‒brain barrier (BBB) dysfunction at an early stage after subarachnoid hemorrhage (SAH) ictus are still unknown. This study elucidated the effect and potential mechanism of intracranial microvasculature-mediated T-cell infiltration on BBB function after SAH.

Methods

Publicly available single-cell RNA sequencing data related to SAH ( https://ngdc.cncb.ac.cn/omix ; Accession No. OMIX006611) were retrieved and analyzed. The dataset was derived from the white matter region of adult male C57BL/6J mice at 1 and 7 days after experimental SAH. The SAH model was induced by endovascular perforation, and experiments were subsequently conducted at 1, 3, 7, and 14

SUBMITTER: Li Y 

PROVIDER: S-EPMC12020083 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature