Chaperone-assisted E3 ligase-engineered mesenchymal stem cells target hyperglycemia-induced p53 for ubiquitination and proteasomal degradation ameliorates self-renewal.

ABSTRACT:

Background

Stem cell therapies may potentially be used in regenerative and reconstructive medicine due to their ability for self-renewal and differentiation. Stressful conditions, such as hyperglycemia, adversely affect stem cell functions, impairing their function and promoting differentiation by opposing self-renewal. The carboxyl terminus of HSP70 interacting protein (CHIP), which is a cochaperone and E3 ligase, maintains protein homeostasis and performs quality control of the cell via ubiquitylation. However, the role of CHIP in regulating stemness remains unknown.

Results

Hyperglycemia downregulated CHIP-induced p53, arrested the cell cycle at the gap (G₁) phase, and promoted the loss of stemness in WJMSCs. Quantitative real-time polymerase chain reaction (qR

SUBMITTER: Ali A 

PROVIDER: S-EPMC12020092 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature