E2F8-induced GRPEL2 promoted colorectal cancer progression via targeting TIGAR.

ABSTRACT:

Background

Colorectal cancer (CRC) is the leading cause for cancer mortality across the world. GRPEL2 is a critical regulator of mitochondria's function with an oncogenic role in different kinds of cancer. The exact function of GRPEL2 -driven mitochondrial regulation and CRC progression have not been elucidated.

Methods

RNA-seq data from TCGA database was analyzed to identify biomarkers and therapeutic targets of CRC. The gene expression profile was validated by quantitative real-time PCR on 68 paired tumor and non-tumor samples from CRC patients. Tumorigenesis regulated by GRPEL2 was tested through EdU staining, Transwell assay, in vivo tumor growth and in vivo metastasis. The function of Mitochondria mediated by GRPEL2 was evaluated by transmission electron microscopy, DCF

SUBMITTER: Song C 

PROVIDER: S-EPMC12020167 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature