Effect of Particulate Matter 2.5 on Primary Gingival Keratinocyte and Human Gingival Fibroblast Cell Lines.

ABSTRACT:

Objective

 Particulate matter 2.5 (PM2.5), an important air pollution particle, has been previously studied for its effects on various normal and cancer tissues. However, research on the impact of PM2.5, specifically on normal cavity tissue, is still limited. This study aimed to assess the effects of PM2.5 on cell vitality, cell cycle, and apoptosis in PGK (normal oral keratinocyte) and HGF (human gingival fibroblast) cell lines.

Materials and methods

 The effect of PM2.5 was examined through cell vitality using the Cell Counting Kit-8 (CCK8) assay, while cell cycle and apoptosis were determined via flow cytometry. Cells incubated with 0.05% dimethyl sulfoxide were used as the negative control.

Results

 In a concentration-dependent manner, PM2.5 inhibited the prolifer

SUBMITTER: Mala S 

PROVIDER: S-EPMC12020600 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature