Expression Distribution of Keratins in Normal and Pathological Corneas and the Regulatory Role of Krt17 on Limbal Stem Cells.

ABSTRACT:

Purpose

This study aimed to compare variations in keratins (KRTs), particularly stress KRTs, under normal and pathological conditions, with a specific focus on investigating the role of KRT17.

Methods

This research examined changes in KRT and limbal stem cell (LSC) markers in normal and various pathological corneas using mRNA and proteomic sequencing data alongside single-cell sequencing data from normal mouse corneas. The effects of the Krt17 recombinant protein and specific small interfering RNA on the clonal formation and proliferation of human limbal epithelial cells (HLECs) were investigated. mRNA sequencing was conducted on Krt17 knockdown HLECs, and the findings were validated using qPCR, immunofluorescence staining, neutrophil chemotaxis, and herpes simplex virus 1 i

SUBMITTER: Wang M 

PROVIDER: S-EPMC12020953 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature