High-Speed Hyperspectral Imaging for Near Infrared Fluorescence and Environmental Monitoring.

ABSTRACT: Hyperspectral imaging captures both spectral and spatial information from a sample but is intrinsically slow. The near infrared (NIR, > 800 nm) is advantageous for imaging applications because it falls into the tissue transparency window and also contains vibrational overtone and combination modes useful for molecular fingerprinting. Here, fast hyperspectral NIR imaging is demonstrated using a spectral phasor transformation (HyperNIR). A liquid crystal variable retarder (LCVR) is used for tunable, wavelength-dependent sine- and cosine-filtering that transforms optical signals into a 2D spectral (phasor) space. Spectral information is thus obtained with just three images. The LCVR can be adjusted to cover a spectral range from 900 to 1600 nm in windows tunable from 50 to 700 nm, which enabl

SUBMITTER: Stegemann J 

PROVIDER: S-EPMC12021105 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature