Pleomorphic adenoma and carcinoma ex-pleomorphic adenoma tumorigenesis: A proteomic analysis.

ABSTRACT:

Objectives

To conduct a comprehensive proteomic analysis of normal salivary gland tissue, pleomorphic adenoma (PA), and carcinoma ex-pleomorphic adenoma (CXPA), and validate the proteomic findings using immunohistochemistry.

Methods

Six normal salivary gland tissues, seven PA and seven CXPA samples underwent laser microdissection followed by liquid chromatography coupled to mass spectrometry. Protein identification and quantification were performed using MaxQuant software. Statistical analysis and functional enrichment were conducted using the Perseus platform and STRING tool, respectively. Immunohistochemistry was used for validation.

Results

Comparative proteomic analysis revealed 2680 proteins across the three tissue types, with 799 significantly altered between gr

SUBMITTER: Zanella VG 

PROVIDER: S-EPMC12021308 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature