Method development and clinical validation of LAMP-CRISPR/Cas12a for rapid detection of respiratory pathogens in children.

ABSTRACT:

Background

Respiratory tract infections pose a substantial health burden, particularly among pediatric populations globally. The timely and accurate identification of pathogens such as Streptococcus pneumoniae (SP) and Mycoplasma pneumoniae (MP) is critical for effective clinical management.

Methods

In this study, a novel diagnostic approach combining loop-mediated isothermal amplification (LAMP) with CRISPR-Cas12a technology was developed for detecting SP and MP in clinical respiratory samples. A total of 23 specimens, including bronchoalveolar lavage fluid and nasopharyngeal swab samples, were assessed to evaluate the feasibility and performance of the method. After nucleic acid extraction, samples underwent LAMP amplification followed by CRISPR-Cas12a-mediat

SUBMITTER: Zhou S 

PROVIDER: S-EPMC12021640 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature