Beyond the loss of beta cells: a quantitative analysis of islet architecture in adults with and without type 1 diabetes.

ABSTRACT:

Aims/hypothesis

The organisation and cellular architecture of islets of Langerhans are critical to the physiological regulation of hormone secretion but it is debated whether human islets adhere to the characteristic mantle-core (M-C) structure seen in rodents. It is also unclear whether inherent architectural changes contribute to islet dysfunction in type 1 diabetes, aside from the loss of beta cells. Therefore, we have exploited advances in immunostaining, spatial biology and machine learning to undertake a detailed, systematic analysis of adult human islet architecture in health and type 1 diabetes, by a quantitative analysis of a dataset of >250,000 endocrine cells in >3500 islets from ten individuals.

Methods

Formalin-fixed paraffin-embedded pancreatic sections (4 μm)

SUBMITTER: Verschueren van Rees N 

PROVIDER: S-EPMC12021988 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature