A novel strategy for Escherichia coli detection in raw beef in combination with click chemistry.

ABSTRACT: Bacteria-assisted click chemistry for enhanced nanozyme-based Escherichia coli (E. coli) detection. The size and shape of the alkyne-modified Fe₃O₄@Au NPs were 110 nm and urchin-like, respectively. The azide-functionalized Au QDs were spherical in shape, and their size was ~5 nm. The absorbance and emission peaks of Fe₃O₄@Au NPs and Au QDs were located at 526 nm and 520 nm, respectively. The nanozymatic activity of both materials significantly improved in the presence of Cu²⁺, E. coli, and 3,3',5,5'-tetramethylbenzidine (TMBZ)/H₂O₂ mixture. Here, the bacterial metabolic product Cu⁺ was utilized to trigger the click reaction between alkyne-modified Fe₃O₄@Au NPs and azide-functionali

SUBMITTER: Alzahrani A 

PROVIDER: S-EPMC12022034 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature