Proteomics approach identifies aqueous humor biomarkers in retinal diseases.

ABSTRACT:

Background

Geographic atrophy (GA) secondary to age-related macular degeneration (AMD) is one of the main causes of blindness in the elderly population, but the molecular pathophysiology is difficult to study due to limited access to retinal tissue. We investigated aqueous humor (AH) as an accessible surrogate for studying retinal pathophysiology.

Methods

We applied affinity-based Olink proteomics on AH samples obtained from 30 non-AMD control, 30 intermediate AMD (iAMD) and 28 GA subjects to identify AH biomarkers associated with GA. Quantile normalization was applied to the Olink data, followed by differential abundance analysis using the limma R package. To contextualize our findings, we cross-referenced the identified proteins to gene expression datasets and AH proteomic

SUBMITTER: Huang K 

PROVIDER: S-EPMC12022036 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature