Virome analysis of field-collected chilli samples reveals diverse viruses.

ABSTRACT:

Background

Chilli (Capsicum annuum L.), an important spice crop, is susceptible to diverse viral infections. Traditional detection methods including PCR and its variants had difficulty in identifying the complete spectrum of viruses, especially in mixed infections. High-throughput sequencing (HTS) has emerged as a successful tool for comprehensive virome analyses, enabling the identification of the known and novel viruses in the infected samples. Using HTS, we investigated virome analyses to identify known and novel viruses in chilli.

Methods

In 2021-22, 19 leaf samples were collected from chili plants in farmer fields in Karnataka, India, showing symptoms such as leaf curling, vein banding, mosaic, mottling, filiform, leathery, dull-colored, and bunchy leaves. Total RNA was

SUBMITTER: Netla VR 

PROVIDER: S-EPMC12023414 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature