A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis.

ABSTRACT:

Background

Alzheimer's disease (AD) is characterized by progressive amyloid beta (Aβ) deposition in the brain, with eventual widespread neurodegeneration. While the cell-specific molecular signature of end-stage AD is reasonably well characterized through autopsy material, less is known about the molecular pathways in the human brain involved in the earliest exposure to Aβ. Human model systems that not only replicate the pathological features of AD but also the transcriptional landscape in neurons, astrocytes and microglia are crucial for understanding disease mechanisms and for identifying novel therapeutic targets.

Methods

In this study, we used a human 3D iPSC-derived neurosphere model to explore how resident neurons, microglia and astrocytes and their interplay are modif

SUBMITTER: Wendt S 

PROVIDER: S-EPMC12023538 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature