Supercritical Fluid-Processed Multifunctional Hybrid Decellularized Extracellular Matrix with Chitosan Hydrogel for Improving Photoaged Dermis Microenvironment.

ABSTRACT: To address the demand for reconstructive procedures in extensive subcutaneous tissue defects and significant dermis matrix loss, vascularized adipose tissue regeneration is essential for maintaining volume after material degradation. Accordingly, a double-crosslinked hydrogel that combines polyethylene glycol (PEG)-crosslinked carboxymethyl chitosan (CMC) with a hybrid decellularized extracellular matrix (dECM) is developed. The dECM, sourced from porcine adipose and cardiac tissues, processed using a supercritical fluid technique (scCO₂-EtOH) retains 1.5-5-fold more angiogenic and adipogenic cytokines than that processed using traditional methods. This hybrid dECM-based filler demonstrates excellent physical properties and injectability, with injection forces being significantl

SUBMITTER: Jeong SH 

PROVIDER: S-EPMC12023828 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature