Effect of Alternative Splicing Euchromatic Histone Lysine Methyltransferase 2 (<i>EHMT2/G9A</i>) on Spermatogenesis in Mongolian Horses.

ABSTRACT: The epigenetic regulation of gene expression through the covalent modification of histones is crucial for developing germline cells. To study the regulatory role of alternative splicing (AS) of euchromatic histone lysine methyltransferase 2 (EHMT2/G9A) in spermatogenesis in Mongolian horses, this study first examines the localization of the EHMT2 gene in testicular support cells and then predicts the higher-order structures of sequences with and without AS. Two types of lentiviral vectors for overexpression were subsequently constructed for the EHMT2 gene, one with AS and one without, to infect support cells. The proliferation and activity of infected cells were measured using CCK8, and the differential expression of spermatogenesis-related genes in the two types of support c

SUBMITTER: Baatar T 

PROVIDER: S-EPMC12024092 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature