Bioactive Lignan Glycosides in Stems of Marsh Rosemary (<i>Rhododendron tomentosum</i>): Non-Targeted Screening and Identification Using Two-Stage Analytical Strategy.

ABSTRACT: Rhododendron tomentosum is a widespread evergreen shrub used in folk medicine due to the high biological activity of its secondary metabolites, including lignans, that has not been sufficiently studied, and overcoming this problem requires advanced analytical techniques. This study proposes a two-stage analytical strategy for non-targeted screening and identification of lignans in plant extracts that involves the detection of specific lignan-related structures by 2D NMR and the establishment of corresponding fragment ions for further mass spectrometry analysis (HPLC-ESI-MS/MS). The polyphenolic fraction of R. tomentosum stem extract was the object of the study. Eight secoisolariciresinol-type lignans (xylosides, glucosides, and rhamnoside), including one previously unknown co

SUBMITTER: Faleva AV 

PROVIDER: S-EPMC12024211 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature