Efficacy of Ceftobiprole and Daptomycin at Bone Concentrations Against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm: Results of a Dynamic In Vitro PK/PD Model.

ABSTRACT: Background: The presence of biofilms and low antimicrobial concentrations in bone tissue make prosthetic joint infections (PJI) difficult to treat. Ceftobiprole (CTO) has a potential role in MRSA PJI. This study evaluated the efficacy of ceftobiprole and daptomycin (DAP) alone and in combination against MRSA biofilms at expected bone tissue concentrations. We assessed whether CTO-DAP outperformed DAP combined with a non-anti-MRSA beta-lactam (cefazolin [CZO]). Methods: A dynamic in vitro PK/PD biofilm model (CDC biofilm reactor) was used to simulate concentrations expected in cortical bone at a standard dosing of DAP (10 mg/kg/24 h), CTO (500 mg/8 h), and CZO (2 g/8 h), and assess performance against a 48-h MRSA biofilm from two clinical isolates that cause PJI (MRSA-1811 and

SUBMITTER: Mancheno-Losa M 

PROVIDER: S-EPMC12024218 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature