Microbiome and Resistome in Poultry Litter-Fertilized and Unfertilized Agricultural Soils.

ABSTRACT: Background: Poultry litter is the main waste of poultry farming and is widely used as an agricultural fertilizer. However, owing to the use of antimicrobials in animal production, it can accumulate antimicrobial residues, antimicrobial-resistant bacteria (ARB), and antimicrobial resistance genes (ARGs). This study aimed to evaluate the impact of poultry litter use on the microbiome and resistome of agricultural soils. Methods: Soil samples from fertilized and unfertilized plots were collected from two horticultural farms that intensively use poultry litter. Microbiome composition was assessed using 16S rRNA sequencing. A culture-dependent method was used to isolate resistant strains on CHROMagar plates supplemented with sulfamethoxazole or ciprofloxacin. ARGs and integrase-en

SUBMITTER: Lopes EDS 

PROVIDER: S-EPMC12024301 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature