A Facile Surface Modification Strategy for Antibody Immobilization on 3D-Printed Surfaces.

ABSTRACT: 3D-printed microdevices have become increasingly important to the advancement of point-of-care (POC) immunoassays. Despite its great potential, using 3D-printed surfaces on the solid support for immunorecognition has been limited due to the non-ideal adsorption properties for many photocurable resins. In this work, we report a simple surface modification protocol that works for diverse commercial photocurable resins, improving ELISAs performed directly on 3D-printed devices. This surface modification strategy involves surface activation via air plasma followed by the one-step incubation of GLYMO-labeled streptavidin. We successfully immobilized biotinylated anti-activin A antibodies on the 3D-printed surfaces and performed the complete ELISA protocol on the 3D-printed surfaces. We demonstr

SUBMITTER: Binkley B 

PROVIDER: S-EPMC12024930 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature