Macrophage-Hosted Porphyromonas gingivalis Is a Risk Factor for Cataract Development.

ABSTRACT:

Purpose

We studied the regulatory association of Porphyromonas gingivalis (PG) and cataracts.

Methods

PCR and FISH assays were used for detecting PG 16s ribosomal RNA genome, Immunofluorescence was for expression of RpgA in anterior capsular epithelium and fibrosis markers in anterior subcapsular cataract (ASC) model. Flow cytometry was for reactive oxygen species and apoptosis. RNA deep sequencing is for differential gene expression analysis.

Results

PG's 16s ribosomal RNA gene is positively in 43.3% (101/233 cases) of aqueous humor (AH) samples of patients with cataracts, which differs from 4.7% (6/127) of PG-positive AH in patients with glaucoma. Diabetic and high myopia cataracts increase PG-positive AH compared with age-related cataracts. No PG is observed in AH

SUBMITTER: Zhang D 

PROVIDER: S-EPMC12025317 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature