Microglia-Derived IL-6 Promotes Muller Glia Reprogramming and Proliferation in Zebrafish Retina Regeneration.

ABSTRACT:

Purpose

Inflammation activates the Jak1-Stat3 signaling pathway in zebrafish Müller glia (MG), leading to their status transition and proliferation following retinal injury. However, the source of Stat3-activating molecules remains unclear. This study aims to explore the expression and function of a Stat3-activating cytokine IL-6 in zebrafish retina regeneration.

Methods

Mechanical retinal injury was induced in adult zebrafish by a needle-poke lesion. Single-cell RNA sequencing (scRNAseq) and PCR were used to determine gene expression. Microglia ablation was performed by using the mpeg1:nsfb-mcherry transgenic zebrafish. Morpholino oligonucleotides, a recombinant zebrafish IL-6 protein and drugs, were used to manipulate IL-6 or Stat3 signaling in the retina. The 5-Ethynyl-2'

SUBMITTER: Xu J 

PROVIDER: S-EPMC12025339 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature