Crizotinib Inhibits Viability, Migration, and Invasion by Suppressing the <i>c-Met</i>/<i>PI3K</i>/<i>Akt</i> Pathway in the Three-Dimensional Bladder Cancer Spheroid Model.

ABSTRACT: We aimed to evaluate the therapeutic potential of crizotinib, a broad-spectrum tyrosine kinase inhibitor against bladder cancer (BC) cells, based on a three-dimensional (3D) cell culture system. After proliferating cell masses (spheroids) using T24 cisplatin-naïve and T24R2 cisplatin-resistant human BC cell lines, the spheroids were exposed to various crizotinib concentrations in order to derive an ideal crizotinib concentration to suppress cell survival, migration, and invasion. Crizotinib suppressed cell proliferation, migration, and invasion in both T24 and T24R2 BC cell lines under a 3D spheroid model, which was more appropriate than the conventional two-dimensional cell culture model. Real-time quantitative polymerase chain reaction analysis revealed a reduced expression of E-cadhe

SUBMITTER: Song B 

PROVIDER: S-EPMC12025888 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature