FRET Visualization of High Mechanosensation of von Willebrand Factor to Hydrodynamic Force.

ABSTRACT: von Willebrand factor (vWF) is a large glycoprotein in the circulation system, which senses hydrodynamic force at vascular injuries and then recruits platelets in assembling clots. How vWF mechanosenses shear flow for molecular unfolding is an important topic. Here, a Förster resonance energy transfer (FRET) biosensor was developed to monitor vWF conformation change to hydrodynamic force. The vWF-based biosensor is anchored on the cell surface, in which the A2 domain is flanked with a FRET pair. With 293T cells seeded into microfluidic channels, 2.8 dyn/cm² of shear force (i.e., 28 μN/cm², or 264.1/s in shear rate) induced a remarkable FRET change (~60%) in 30 min. A gradient micro-shear below 2.8 dyn/cm² demonstrated FRET responses positively related to fl

SUBMITTER: Ouyang M 

PROVIDER: S-EPMC12026062 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature