Achievement of 15-Minute Adaptive PCR Benchmark with 1370 nm Laser Heating.

ABSTRACT: In low-resource and point-of-care settings, traditional PCR often faces challenges of poor sample preparation, adverse environmental conditions, and long assay times. We have previously described a laboratory-based instrument to achieve "adaptive" PCR, a PCR thermocycling control system that replaces preset cycling times and temperatures with the optical monitoring of added L-DNA stereoisomers matching the sequences of the reaction primers and target. These L-DNA biosensors directly monitor DNA hybridization, compensating for ambient environmental conditions and poor sample preparation. This report describes instrument simplifications and a comparative evaluation of both direct photothermal and plasmonic laser heating to reduce the assay time to 15 min. Instrument performance was assessed

SUBMITTER: Spurlock N 

PROVIDER: S-EPMC12026111 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature