A New Model to Investigate the Action of Radiation and Cigarette Smoke on Head and Neck Cancer Cells.

ABSTRACT: Background/Objectives: Smokers are at an increased risk of developing mucosal head and neck squamous cell cancers (HNSCCs) and have a worse prognosis when treated. The cellular and molecular mechanisms underlying the latter has not been established. We therefore developed an in vitro model to investigate the effects of radiation and smoking on mucosal HNSCCs. FaDu hypopharyngeal cancer cells were subjected to daily fractionated radiation and cultured with and without cigarette-smoke-exposed media. Methods: The cells were characterised using assays for tumour sphere formation, proliferation, migration, invasion, CD44 and ALDH expression, and next generation sequencing. We also evaluated CD44 and ALDH1 expression in patient tumour samples. Results: Radiation and smoking

SUBMITTER: Lopes Floro K 

PROVIDER: S-EPMC12026225 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature