Ultrasensitive CRISPR/Cas12a-Based System for Detection of <i>Bla</i>_OXA-1 Gene in Antibiotic-Resistant Microorganisms.

ABSTRACT: The bla_OXA-1 gene encodes an oxacillin-hydrolyzing beta-lactamase of extended-spectrum beta-lactamase (ESBL)-producing microorganisms. The bla_OXA-1 gene is found in the resistomes of some Enterobacteriaceae, Morganellaceae, Pasteurellaceae, Moraxellaceae, Aeromonadaceae, Pseudomonadaceae, Yersiniaceae, and Vibrionaceae. Most ESBL detection methods, including those to detect OXA-1-producing microorganisms, are time-consuming, and require specialized equipment and qualified personnel. Here, we report a new CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas12a-based detection assay coupled with polymerase chain reaction (PCR) to sensitively detect OXA-1-bearing microorganisms. The PC

SUBMITTER: Tyumentseva M 

PROVIDER: S-EPMC12026237 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature