Secretory Production of Plant Heme-Containing Globins by Recombinant Yeast via Precision Fermentation.

ABSTRACT: Leghemoglobin (LegHb) is a plant-derived heme-containing globin found in the root nodules of legumes like soybean that can be used as a food additive for red color and meaty flavor as a plant-based meat alternative. However, conventional extraction methods face challenges of low yield and high costs. To address this issue, precision fermentation with recombinant microorganisms has been applied for the sustainable large-scale production of plant leghemoglobins. This study attempted the production of plant legHbs using recombinant yeast strains, Saccharomyces cerevisiae and Komagatella phaffii. The plant legHb genes were identified from the genome of legumes such as soybean, chickpea, mung bean and overexpressed in yeast via extracellular secretion by the signal peptide and ind

SUBMITTER: Bae HN 

PROVIDER: S-EPMC12026525 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature