Warfarin Pharmacogenomics: Designing Electrochemical DNA-Based Sensors to Detect <i>CYP2C9</i>*2 Gene Variation.

ABSTRACT:

Background/objectives

The CYP2C9 enzyme is involved in the metabolism of warfarin. The CYP2C9 gene harbors several single-nucleotide polymorphisms (SNPs), including CYP2C9*2 (rs1799853), which is known to affect warfarin's therapeutic response. So, it is important to develop analytical tools capable of genotyping these SNPs to adjust warfarin's therapeutic outcomes. In this work, an electrochemical DNA-based sensor was constructed and optimized for the detection of the CYP2C9*2 polymorphism.

Methods

Using bioinformatic database platforms, two 71 base pair DNA target probes with the polymorphic variants A and G were chosen and designed. A DNA-based sensor was composed by mercaptohexanol and the CYP2C9*2 DNA capture probe in a self-assembled monolay

SUBMITTER: Barbosa T 

PROVIDER: S-EPMC12027050 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature