Dynamics of IgM and IgA Antibody Response Profile Against <i>Vibrio cholerae</i> Toxins A, B, and P.

ABSTRACT: The first immune response controls many bacterial and viral inflammatory diseases. Oral immunization with cholera toxin (CT) elicits antibodies and can prevent cholerae in endemic environments. While the IgG immune response to the toxin is well-documented, the IgA and IgM epitopes responsible for the initial immune reaction to the toxin remained uncharted. In this study, our objective was to identify and characterize immunologically and structurally these IgA and IgM epitopes. We conducted SPOT synthesis to create two libraries, each containing one hundred twenty-two 15-mer peptides, encompassing the entire sequence of the three chains of the CT protein. We could map continuous IgA and IgM epitopes by testing these membrane-bound peptides with sera from mice immunized with an oral vaccine

SUBMITTER: De-Simone SG 

PROVIDER: S-EPMC12027190 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature