Light-Controlled Synthetic Communication Networks via Paired Connexon Nanopores.

ABSTRACT: Living cells employ dynamic networks for intercellular communication and cooperation, leading to tissue-wide activity. One emerging challenge in the field of bottom-up synthetic biology is emulating such sophisticated behaviors in liposome-based synthetic cells (SCs). Fabricating communication networks in lipid bilayer-based SCs remains a challenge as signaling molecules must transit through two consecutive membranes to transfer information between different SCs. Here, we address this obstacle by engineering connexin channels that directly connect the lumens of adhering SC membranes. We focus on orthogonal channel-forming connexins, namely connexin 43 and connexin 32, and re-design their channel activity to be UV- and near IR-responsive, respectively. By combining engineered connexins into

SUBMITTER: Sihorwala AZ 

PROVIDER: S-EPMC12027324 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature