Optimizing the Methodology for Antioxidant Activity Analysis of Manuka Honey.

ABSTRACT: Manuka honey (MH) is increasingly recognized for its potent antioxidant properties, making it a promising functional food ingredient. However, discrepancies in assessment methods have impeded the standardization of its antioxidant capacity. This study compared DPPH, ABTS, and cellular antioxidant activity (CAA) assays under varying conditions to identify the most reliable approach for assessing MH's antioxidant properties. The results showed that the reaction temperature for the chemical method, setting it at 37 °C, enhanced the antioxidant capacity of MH. For the cellular assay, we optimized honey concentration, injury duration, damage model, and cell model. The result showed that sugar-reduced MH achieved the same high efficiency as the chemical method. A stable cellular assay method was

SUBMITTER: Zhou X 

PROVIDER: S-EPMC12027450 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature