<i>Cis</i>-Regulation of the <i>CFTR</i> Gene in Pancreatic Cells.

ABSTRACT: Genome organization is essential for precise spatial and temporal gene expression and relies on interactions between promoters and distal cis-regulatory elements (CREs), which constitute ~8% of the human genome. For the cystic fibrosis transmembrane conductance regulator (CFTR) gene, tissue-specific expression, especially in the pancreas, remains poorly understood. Unraveling its regulation could clarify the clinical heterogeneity observed in cystic fibrosis and CFTR-related disorders. To understand the role of 3D chromatin architecture in establishing tissue-specific expression of the CFTR gene, we mapped chromatin interactions and epigenomic regulation in Capan-1 pancreatic cells. Candidate CREs are validated by luciferase reporter assay and CRISPR knock-out.

SUBMITTER: Blotas C 

PROVIDER: S-EPMC12027686 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature