Rapid and Economic Baculovirus Titer Determination Using a Novel Transgenic Sf9-QE Cell Line.

ABSTRACT: A baculovirus expression system (BES) for the production of recombinant proteins requires rapid and easy virus titer determination. In this study, a novel direct titration method was developed using a novel Sf9-QE cell line to easily and economically determine virus titers in a short time. This direct titration method can determine virus titers by directly counting the initially infected cells. This method requires the rapid identification of the initial virus-infected cells. The genome of Sf9-QE cells, which fluoresce upon virus infection, was found to contain at least seven copy numbers of the enhanced green fluorescent protein (EGFP) transgene. This result suggests that Sf9-QE cells in the early stages of virus infection can be identified by the high expression of EGFP. It was also show

SUBMITTER: Moon HJ 

PROVIDER: S-EPMC12028008 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature